Liverpool midfielder, Jordan Henderson will captain England against Spain on Tuesday, with Wayne Rooney injured.
Wayne Rooney and left back Ryan Bertrand are injury doubts for the year-ending friendly as they were the only players absent from training on the eve of the meeting with Spain at Wembley.
Having started the previous game – a 0-0 draw away to Slovenia – on the bench, forward Rooney returned to lead the side in Friday’s World Cup qualifying win over Scotland.
He then missed training with a knee injury on Monday and later pulled out of the squad.
“Both are being assessed. They had minor issues this morning, so we’ll have a look at them as the game goes on,” said interim manager, Gareth Southgate, on Monday.
“Obviously, they weren’t able to train, so they must be a doubt, but until we look at them we won’t know for sure.”
Henderson, 26, captained England in their 0-0 draw in Slovenia in October when Rooney was dropped to the bench.
“I don’t think we can have just one captain,” Southgate said.
“We have several leaders. Too much responsibility has been on Wayne – we need to share that. Once you have a team of those leaders you can have a lot of success.”
Tottenham striker, Harry Kane will also not feature against Spain, having been released from the squad over the weekend as he recovers from injury.
The 23-year-old damaged an ankle against Sunderland on 18 September and only returned for 73 minutes of action against Arsenal on 6 November before joining up with the England squad.
He was an unused substitute for the 3-0 win over Scotland at Wembley.