At least 18 people died when two buses collided head-on Wednesday along the Abuja-Lokoja Highway.
Nine others sustained serious injuries, a spokesperson of the Federal Road Safety Commission (FRSC) said.
The accident happened along the Kwali-Gwagwalada road early on Wednesday, when a public bus lost control and rammed into another bus travelling in the opposite direction.
All but one of the 18 passengers of the first bus, including the driver, died on the spot.
A total of 96 people, including 10 children, were involved in the crash.
The FRSC has urged Nigerian motorists to avoid unnecessary night journeys.
The Kwali-Gwagwalada-Lokoja route, linking the north to the rest of the country, is considered particularly dangerous as many people lose their lives here each year.
The road is being expanded to make it safer, but construction work has dragged on for years.